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KMID : 0378019710140010106
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New Medical Journal
1971 Volume.14 No. 1 p.106 ~ p.116
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The Effect of T-Cain on the Cental Nervous System-An Experimental Study
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Abstract
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To determine the possible histotoxic effect of T-Cain(p-butylaminobenzoyl-diethylamino-ethanol hydro￢chloride, a home-made spinal anesthetic) on the central nervous system, a cytological, biochemical and histological study was performed.
Anesthesia of 25 unpremedicated mongrel dogs (weighing 7.5-11.5 kg) was induced with the injection of Pentothal sodium (Abbott, 15-20 mg/kg) into the foreleg cephalic vein and maintained with endotra￢cheal nitrous oxide-oxygen in.semiclosed circle absorption system. Respiration was assisted or controlled as necessary throughout the procedure.
Following the anesthetic induction, femoral artery was cannulated with a polyethylene catheter and arterial pressure was continuously recorded. through the Statham transducer on the Twin-Viso (Sanborn) at the paper speed of 2.5 or 25 mm/sec. Lead ti ECG was also recorded simultaneously.
The animals were then turned to prone position for lumbar laminectomy. The spinal dura was incised and spinal cord exposed under direct vision. Cerebrospinal fluid was aspirated for cytological (red blood cells, lymphocytes, and polymorphonuclear leukocytes) and biochemical (sugar and protein) studies.
Of 25 dogs five were used as control. Physiological saline solution (0.08 ml/kg) was injected into the subarachnoid space in 10 dogs (saline group). In another 10 dogs (T-Cain group), 0.08 ml/kg of 0.5°% T￢Cain solution in 10% D/W was injected intrathecally. In all groups the cytological and biochemical studies were repeated one hour and seven days after the initial experiment.
Arterial hypotension and the lack of motor response to painful stimuli to the hindlegs upon recovery from general anesthesia were considered as the indication of having established sufficient spinal anesthesia.
On the seventh post-laminectomy day all animals were anesthetized with Nembutal (Vitarine), surgical wound was reopened, and the aforementioned studies in addition to biopsies of the lumbar spinal cord, leptomeninges, and dura were performed.
All animals received daily intramuscular injnctions of crystalline procaine penicillin G, 400, 000 units, and dihydrostreptomycin sulfate, 0.5 gm prophylactically throughout the experiment.
The results are as follows:
Except for arterial hypotension nad rise in protein content of the cerebrospinal fluid in the T-Cain
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